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| Okamura, Masashi; Yokoyama, Naoaki; Takabatake, Noriyuki; Okubo, Kazuhiro; Ikehara, Yuzuru; Igarashi, Ikuo; 横山, 直明; 五十嵐, 郁男. |
| In the present study, the subcellular localization of the host red blood cell (RBC) membrane components, the alpha 2-3-linked sialic acid (SA) residues and the lipid bilayer, was observed during the asexual growth of Babesia bovis using two erythrocyte probes, the SA-specific lectin (MALII) and the lipophilic fluorescent (PKH2) probes, respectively. In confocal laser scanning microscopy with MALII, the SA residues on the surface of parasitized RBCs appeared to accumulate into the intracellular parasites as the parasites matured as well as to remain on the surface of extracellular parasites. Furthermore, when PKH2-labeled RBCs were infected with B. bovis, PKH2 signals were also observed around both the intracellular and the extracellular parasites,... |
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Palavras-chave: Babesia bovis (B. bouis); SA; MALII; RBC; RT; PBS; Babesia equi (B. equi); PV. |
| Ano: 2007 |
URL: http://ir.obihiro.ac.jp/dspace/handle/10322/1040 |
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| Nóbrega,Andréa Martins da; Alves,Eloisa Nunes; Presgrave,Rosaura de Farias; Costa,Rodrigo Netto; Delgado,Isabella Fernandes. |
| In an attempt to build the evaluation strategies to assess the human eye irritation, a reassessment of some in vitro tests is necessary, particularly concerning the non-irritants, mild and moderate irritants. Thus, the correlations between results obtained from the Draize test with the in vitro methods HET-CAM (Hens Egg Test-Chorion Allantonic Membrane) and RBC haemolysis assay to assess the ocular irritancy potential of 20 eye drops were examined. Parameters such as accuracy (%) and specificity (%) were determined. All results were correlated with RBC correctly with the results obtained in the Draize test. The HET-CAM presented four false-positive results, showing a tendency of data overestimation. Despite the high specificity provided mainly by the RBC,... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Alternative methods; Eye irritation; Draize Test; Low-irritant; HET-CAM; RBC. |
| Ano: 2012 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132012000300008 |
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| García Alonso, Oscar. |
| Al ser evaluado el proyecto para la Producción de Anturio (Anthurium andreanum), en el municipio de Cuitláhuac, Veracruz, en la forma tradicional, se obtuvo un VAN de $132,986, una Relación Beneficio-Costo (B/C) de 1.07, una Relación Beneficio- Inversión Neta (N/K) de 1.274 y una Tasa Interna de Retorno (TIR) de 17%, por lo cual se llega a la conclusión de la factibilidad del proyecto. Al ser evaluado con la técnica de Opciones Reales bajo los escenarios de expansión, contracción, abandono y Opción estratégica, se obtuvieron los siguientes resultados: $169,209.26, $147,894.73, $145,798.53, $184,038.33; respectivamente para cada una de los escenarios, por lo que se determina que los escenarios más importantes son el de Opción estratégica y la opción de... |
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Palavras-chave: Anthurium andreanum; VAN; RBC; TIR; RBIN; Opciones reales; Real options; Economía; Maestría. |
| Ano: 2013 |
URL: http://hdl.handle.net/10521/1848 |
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| Bhattacharjee,Debasish; Das,Suchismita. |
| ABSTRACT Light and scanning electron microscopic studies of erythrocytes of Channa punctata exposed to two sublethal doses of lindane over a period of 21 days revealed myriads of anomalies. High frequency of micronucleus appeared progressively in the erythrocyte, ranging from 3.8-7.5%, when monitored weekly for 21 d at 0.25 g/L and 1.0-3.0% at 0.025 g/L lindane. Prominent structural anomalies of erythrocytes included abnormal shapes, vacuolation, membrane invagination and disintegration. At higher dose and at later duration, a unique tendency of abnormal cells to stick together was observed. It could be concluded that commercial formulation of lindane could induce genotoxicity and structural anomalies in the erythrocytes of fish. |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: RBC; SEM; Light microscopy; Micronucleus. |
| Ano: 2017 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132017000100449 |
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